![]() ![]() Green sectors of Thf1-disrupted leaves contain some chloroplasts that form organized thylakoid membranes, indicating that an inefficient compensatory mechanism supports thylakoid formation in the absence of Thf1. Nongreen sectors of variegated leaves lacking Thf1 expression contain plastids that accumulate membrane vesicles on the interior and lack organized thylakoid structures. Disruption of the Thf1 gene via T-DNA insertion results in plants that are severely stunted with variegated leaf patterns. Transcript levels for Thf1 are induced in the light and decrease under dark conditions, paralleling profiles of light-regulated nuclear genes involved in chloroplast function. This gene is conserved in oxygenic photoautotrophs ranging from cyanobacteria to flowering land plants. The Arabidopsis Thf1 gene encodes an imported chloroplast protein, as shown by in vitro import and localization of a Thf1-green fluorescent protein fusion product in transgenic plants. ![]() The Arabidopsis (Arabidopsis thaliana) Thylakoid formation1 (Thf1) gene product is shown here to control an important step required for the normal organization of these vesicles into mature thylakoid stacks and ultimately for leaf development. Development of thylakoid membranes depends upon the transport of membrane vesicles from the chloroplast inner envelope and subsequent fusion of vesicles within the interior of the plastid.Songs from the Apple Music catalog cannot be burned to a CD. iTunes-compatible CD or DVD recorder to create audio CDs, MP3 CDs, or backup CDs or DVDs.Internet connection to use Apple Music, the iTunes Store, and iTunes Extras.Screen resolution of 1024x768 or greater 1280x800 or greater is required to play an iTunes LP or iTunes Extras.To play 1080p HD video, a 2.4GHz Intel Core 2 Duo or faster processor, 2GB of RAM, and an Intel GMA X4500HD, ATI Radeon HD 2400, or NVIDIA GeForce 8300 GS or better is required.To play 720p HD video, an iTunes LP, or iTunes Extras, a 2.0GHz Intel Core 2 Duo or faster processor, 1GB of RAM, and an Intel GMA X3000, ATI Radeon X1300, or NVIDIA GeForce 6150 or better is required.To play standard-definition video from the iTunes Store, an Intel Pentium D or faster processor, 512MB of RAM, and a DirectX 9.0–compatible video card is required.PC with a 1GHz Intel or AMD processor with support for SSE2 and 512MB of RAM. ![]()
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